Under the direct supervision of senior research staff in the Melton Lab, the research assistant will support experiments focused on characterizing beta cells derived from in vitro directed differentiation of iPS and/or genetically modified cell lines. The research assistant will support gene editing studies with a focus on designing and generating constructs for gene targeting/editing in ES cells, introduction of plasmids into pluripotent stem cells, screening and endocrine differentiation of edited clones. Research assistants are expected to independently collect and record experimental data and update written protocols and methods based on findings.
Duties and Responsibilities:
Culture human pluripotent stem cells including maintenance, expansion and differentiation to various lineages.
Assist with establishing a standardized tissue culture protocol in the context of directed differentiation of iPS cells or genetically modified lines into pancreatic beta cells and instruct others on the standardized differentiation protocol.
Generate gene-edited lines using CRISPR/Cas9 and related editing techniques for validation/follow-up studies from whole genome screens.
Prepare single-cell and bulk RNA sequencing libraries
Handle downstream assays such as immunofluorescence staining for microscopy, flow cytometry and ELISAs.
Regularly update Melton lab researchers with experimental results.
Carry out laboratory maintenance including cleaning equipment, ordering, and keeping an inventory of laboratory samples.
Complies with all safety, biohazard, and Harvard institutional rules.
This is a grant-funded two-year term position with the possibility of extension based on satisfactory performance and the continued availability of funding
May be required to work with and handle lab-based animals. May be required to lift, move and transport related laboratory equipment. May be required to work with a variety of hazardous materials.
All formal written offers will be made by FAS Human Resources.
We invite individuals with diverse backgrounds, experiences and abilities to be a part of our community
Salary Grade: 052
Union: 55 - Hvd Union Cler & Tech Workers
At least two years of college level course work in biology or related field.
At least one year of related work experience in a biological research laboratory.
Bachelor's degree in biology or closely related field is strongly preferred as is two years related work experience, including culturing (maintenance, expansion and differentiation) of human pluripotent stem cells.
Attention to detail and self-motivated rigor as well as excellent communication, organization, and time management skills, are essential components of this position.
High level of initiative and motivation and ability to work independently and in a collaborative, team-oriented environment is expected.
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
Internal Number: 50431BR
About Harvard University Faculty of Arts and Sciences
Harvard University is devoted to excellence in teaching, learning, and research, and to developing leaders in many disciplines who make a difference globally. The University, which is based in Cambridge and Boston, Massachusetts, has an enrollment of over 20,000 degree candidates, including undergraduate, graduate, and professional students. Harvard has more than 360,000 alumni around the world. The University has twelve degree-granting Schools in addition to the Radcliffe Institute for Advanced Study, offering a truly global education. Established in 1636, Harvard is the oldest institution of higher education in the United States.