Under the direction of senior research staff, research assistant will support studies focused on characterizing the cells derived from in vitro differentiation of stem cells towards pancreatic beta cells and other endocrine cell types.
More specifically, research assistant will:
Use molecular biology techniques (PCR, cloning, sequencing) to generate construct for gene knock-out and knock-in via lentiviral delivery
Assist with tissue culture in the context of directed differentiation stem-cell derived pancreatic beta cells;
Create genetically modified stem cell lines and assess the phenotypes of these genetic modifications;
Operate inDrops microfluidic sequencing platform to encapsulate single cells in droplets;
Prepare RNA sequencing libraries from inDrops-encapsulated single cells and other bulk libraries;
Carry out in-depth quality control of sequencing libraries;
Plan and coordinate sequencing process with core facility and Melton lab staff;
Handle immunofluorescence staining for microscopy and flow cytometry for validation of single cell RNA sequencing findings;
Collect and record experimental data, and update written protocols and methods based on findings;
Regularly update Melton lab researchers with experimental results;
Carry out laboratory maintenance including cleaning equipment, ordering, and keeping an inventory of laboratory samples.
This position offers the opportunity to engage in cutting edge research while working with leaders in the field of stem cell biology and diabetes. The position is particularly focused on supporting work tied to human genetic perturbations and their effect in the context of differentiation.
This is a grant-funded one-year term position with the possibility of extension based on satisfactory performance and the continued availability of funding.
May be required to work with and handle lab-based animals May be required to lift, move and transport related laboratory equipment. May be required to work with a variety of hazardous materials.
Salary Grade: 052
Union: 55 - Hvd Union Cler & Tech Workers
Minimum of 2 years of college level coursework and lab training in cellular and molecular biology. Experience with molecular biology (PCR, etc.) and cell culture (mammalian cell culture) are required.
BS in biology or related field preferred
At least one year related work experience (relevant course work may count towards experience).
Demonstrated abilities in basic laboratory techniques helpful, but not required
Exposure to applicable computer technologies, including specific software applications, may be required
Highly organized and able to multitask
Ability to think creatively to solve problems
Self-motivated with excellent communication skills
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
Internal Number: 47763BR
About Harvard University Faculty of Arts and Sciences
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