This is a unique opportunity to engage in cutting edge research while working with leaders in the field of stem cell biology and diabetes. Under the direction of senior research staff in the Melton Lab, the research assistant will support studies focused on characterizing the cells derived from in vitro differentiation of stem cells towards pancreatic beta cells with a particularly focus on work tied to single-cell RNA sequencing.
Duties and Responsibilities:
Assist with tissue culture in the context of directed differentiation of stem cells into pancreatic beta cells
Prepare human cell cultures for single-cell RNA sequencing
Operate inDrops microfluidic sequencing platform to encapsulate single cells in droplets
Prepare RNA sequencing libraries from inDrops-encapsulated single cells and other bulk libraries
Carry out in-depth quality control of sequencing libraries
Plan and coordinate sequencing process with core facility and Melton lab staff
Handle immunofluorescence staining for microscopy and flow cytometry for validation of single cell RNA sequencing findings
Collect and record experimental data, and update written protocols and methods based on findings
Instruct others on protocols and use of inDrops microfluidic sequencing platform
Regularly update Melton lab researchers with experimental results
Carry out laboratory maintenance including cleaning equipment, ordering, and keeping an inventory of laboratory samples
Complies with all safety, biohazard, and Harvard institutional rules
This is a one year grant funded term position with the possibility of extension based on satisfactory performance and continued availability of funding.
May be required to lift, move and transport related laboratory equipment.
May be required to work with a variety of hazardous materials. May be required to work with and handle animals.
Salary Grade: 052
Union: 55 - Hvd Union Cler & Tech Workers
Education: College level coursework in biological sciences or equivalent work experience are required.
Bachelor's degree in biology or closely related field is strongly preferred as is two years related work experience in a biological research laboratory. Good organizational skills, excellent attention to detail and self-motivated rigor are essential components of this position. High level of initiative and motivation and ability to work independently and in a collaborative, team-oriented environment is expected. Experience with RNA and next-generation sequencing library construction is a plus.
EQUAL OPPORTUNITY EMPLOYER: We are an equal opportunity employer and all qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability status, protected veteran status, or any other characteristic protected by law.
About Harvard University Faculty of Arts and Sciences
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